Abstract: Application of electrodialysis technology to desalination and separation of complex amino acids was necessarily demonstrated by analyzing advantages and disadvantages of desalination and separation of complex amino acids. Effect of electrodialysis technology for desalination and separation of complex amino acids was tested. The correlation between concentration and conductivity of inorganic salts in complex amino acids solution was determined. The results show that concentration and conductivity of inorganic salts depend on different diverse times and high and/or low concentration-room in mother solution. Their liner relationship is K=2.957 3c(NaCl)-0.205 4, and the corresponding coefficient is 0.967 3. It is thus inferred that electrodialysis technology could effectively change the concentration in the concentrating desalination rooms and the separation of inorganic salts and further purification of complex amino acids could be obtained.